All Things Chromatography

An Apple A Day - minus the patulin - how Thin Layer Chromatography provides faster, cheaper answers

Here's an excerpt from Scirus.com about using Thin Layer apple with patulinChromatography for the analysis of patulin in apple juice (emphasis added) :

Researchers from Brazil developed a rapid, simple and economical method using thin-layer chromatography quantification via fluorescence images from a UV lamp, for the determination of patulin in apple juice concentrate

Why it matters

According to the authors, patulin is a mycotoxin produced by certain species of Penicillium, Aspergillus and Byssochlamys. In previous research studies, liquid chromatography has been used for patulin determination in clear and cloudy apple juices, apple puree and apple-based products intended for infants. Methods such as gas and liquid Thin Layer Chromatography detects patulinchromatography have very low detection limits, but both techniques require sophisticated and costly equipment, extensive cleanup procedures and high purity solvents. Thin-layer chromatography (TLC) is a fast, cheap and efficient method of separation and identification of many mycotoxins. By coupling a two-dimensional charge coupled device with thin-layer chromatography, the entire TLC can be imaged in a single exposure, yielding rapid quantification in shorter analysis time than slit scanning densitometers.

 Click Here for the full Abstract.

Topics: biotechnology, patulin, Thin Layer Chromatography, test