All Things Chromatography

Separation of Lipids via Thin Layer Chromatography

This post comes to us from "Biochem write up"

Intro:
The aim of this practical is to carry out TLC in which the substances we wish to separate are absorbed onto the thin layer. Lipids are determined by isolation and the ability to purify the substance. Due to the way the substances interact with the matrix in different ways we are able to seperate them. Substances which interact strongly with the matrix but not with the solvent will move very slowly and those soluble in the solvent will dissolve easily and be carried along the solvent.
thin layer chromatography phospholipids
Egg yolk contains trioleine, cholesterol palmitate and phosphatidylethonolamine, The most polar of the three elements is the second element. It hardly moves up the TLC because of the presence of several polar groups; phosphate groups, amine groups and several oxygens , and cholesterol palmitate being the most non polar. 

Learn More! Click Here for a Free Download of our mini-application note: Analysis of Phospholipids by One-Dimensional Thin-Layer Chromatography 

Topics: Separation, Scientist, Thin Layer Chromatography, Blog, lipids

Free Book: “Interfacial Phenomena in Chromatography"

Our friends at "Scientist-At-Work" are offering the book “Interfacial Phenomena in Chromatography" as a free download!

“Interfacial Phenomena in Chromatography Interfacial Phenomena in Chromatography presents a combination of chromatographic theory, numerical simulation and experimental data. The text covers the interaction and size exclusion methods of separation, identification and characterization of substances in solution. It provides practical information and analysis on the most effective mechanisms of interfacial chromatography, along with its expanding possibilities for biomedical, industrial and environmental applications.

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Topics: Scientist, chromatography, Thin Layer Chromatography, Blog

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Topics: Analtech, video, Announcements, chromatography, Thin Layer Chromatography, Blog

From another great Blog: PRACTICAL 4: SEPARATION OF LIPIDS VIA THIN LAYER CHROMATOGRAPHY

We love to see Chromatography written about all over the place. So whenever something good is brought to our attention, we want to pass it along to you.

This is from Biochemistry Practicals:

In this practical the lipids present in egg yolk were separated through absorption chromatography using a TLC plate. The concept is similar to paper chromatography, a plate containing a thin hydrophilic layer called a matrix is used, and a non polar solvent is allowed to move up the plate through capillary action. Thus more polar lipids will move more slowly than non polar lipids.

Click Here to read the full post.

Topics: chromatography, Thin Layer Chromatography, Blog