All Things Chromatography

Celebrating the birth of the father of Chromatography

Mikhail Semyonovich Tsvet was born on this day in 1872.Tsvet

Here's an excerpt from Discoveries in Medicine about the significance of Tsvet's work:

The first chromatograph was invented by Russian botanist Mikhail Semenovich Tsvett (1872-1919). While working in Poland, Tsvett was looking for a method of separating a mixture of plant pigments (tints) which are chemically very similar to each other. To isolate different types of chlorophyll, he trickled a mixture of dissolved pigments through a glass tube packed with calcium carbonate powder. As the solution washed downward, each pigment stuck to the powder with a different degree of strength, creating a series of colored bands. Each band of color represented a different substance. Tsvett referred to the colored bands as a chromatogram. He also suggested that the technique (now called adsorption chromatography) could be used to separate colorless substances.

Although Tsvett published a report of his work in the early 1900s, chemists paid very little attention to it. There were a few reasons for ignoring the work. First, the report was written in Russian, which few Western chemists of the time read. Second, the technique may have seemed too simple to chemists who were used to relying on lengthy extraction, crystallization, or distillation processes to separate mixtures. Within a few years, Tsvett's technique was rediscovered. The rediscovery was by the German organic chemist Richard Martin Willstatter (1872-1942), who was also studying chlorophyll. By introducing chromatography to Western European scientists, Willstatter helped establish one of the most versatile analytical techniques known to chemistry.

Join us today as we honor the work of Mikhail Semyonovich Tsvet

Topics: botany, chromatography, Tsvet